LOCUS       Exported                3580 bp ds-DNA    circular SYN 17-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0059_sensor_pluxRstRBS-sfGFP
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Daniel Gerngross
  TITLE     Direct Submission
  JOURNAL   Exported 17 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      1..126
                     /note="T0"
     terminator      27..121
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     misc_feature    127..196
                     /note="spacer (B0040)"
     promoter        197..251
                     /note="lux rR (R0062)"
     RBS             260..271
                     /note="RBS (B0034)"
     CDS             278..1003
                     /codon_start=1
                     /note="sfGFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRP*"
     terminator      1006..1122
                     /note="T1"
     terminator      1015..1101
                     /gene="Escherichia coli rrnB"
                     /note="T1"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1129..1148
                     /note="AB179"
     primer_bind     1314..1333
                     /note="AB5"
     rep_origin      1467..2294
                     /direction=RIGHT
                     /note="p15A"
     rep_origin      1715..1717
                     /direction=RIGHT
                     /note="P15 ori from pACYC184"
     rep_origin      complement(1718..2262)
                     /direction=LEFT
                     /note="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     primer_bind     2366..2385
                     /note="AB180"
     oriT            2396..2504
                     /note="incP origin of transfer"
     CDS             complement(2660..3475)
                     /note="KamR"
ORIGIN
        1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg
       61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      121 ccaagaaggt tctgttaagt aactgaaccc aatgtcgtta gtgacgctta cctcttaaga
      181 ggtcactgac ctaacaacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat
      241 agtcgaataa atactagaga aagaggagaa atactagatg agcaaaggag aagaactttt
      301 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
      361 tgtccgtgga gagggtgaag gtgatgctac aaacggaaaa ctcaccctta aatttatttg
      421 cactactgga aaactacctg ttccgtggcc aacacttgtc actactctga cctatggtgt
      481 tcaatgcttt tcccgttatc cggatcacat gaaacggcat gactttttca agagtgccat
      541 gcccgaaggt tatgtacagg aacgcactat atctttcaaa gatgacggga cctacaagac
      601 gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaagggtat
      661 tgattttaaa gaagatggaa acattcttgg acacaaactc gagtacaact ttaactcaca
      721 caatgtatac atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg
      781 ccacaacgtt gaagatggtt ccgttcaact agcagaccat tatcaacaaa atactccaat
      841 tggcgatggc cctgtccttt taccagacaa ccattacctg tcgacacaat ctgtcctttc
      901 gaaagatccc aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg
      961 gattacacat ggcatggatg agctctacaa aaggccttaa taaattaaag gcatcaaata
     1021 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1081 gctctcctga gtaggacaaa tccgccgccc tagacagctg ggcgcgccaa atacgcccgg
     1141 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
     1201 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta
     1261 ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc
     1321 tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt
     1381 ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg
     1441 ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt
     1501 cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg
     1561 tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
     1621 cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata
     1681 cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg
     1741 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
     1801 gataccaggc gtttcccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt
     1861 taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt
     1921 ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc
     1981 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac
     2041 cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt
     2101 taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg
     2161 ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg
     2221 ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat
     2281 cagataaaat atttctagca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2341 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gctagggccg gcctacggcc
     2401 agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga
     2461 aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga
     2521 tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg
     2581 atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaagg
     2641 acccttgtcc aattaattat tagaaaaatt catccagcat cagatgaaat tgcagtttgt
     2701 tcatatccgg attatcaatg ccatatttct gaaacagacg tttttgcagg ctcgggctaa
     2761 attcgcccag gcagttccac agaatggcca gatcctgata acgatccgca atgcccacac
     2821 ggcccacatc aatgcagcca atcagtttgc cttcatcgaa aatcaggtta tccaggctaa
     2881 aatcgccgtg ggtcaccacg ctatccgggc taaacggcag cagtttatgc atttctttcc
     2941 acacctgttc caccggccag ccgttacgtt catcatcaaa atcgctcgca tccaccaggc
     3001 cgttgttcat acggctctgc gcctgggcca gacgaaacac acgatcgctg ttaaacgggc
     3061 agttgcacac cggaatgcta tgcagacgac gcagaaacac ggccagcgca tccacaatgt
     3121 tttcgccgct atccggatat tcttccagca cctgaaacgc ggttttgccc ggaatcgcgg
     3181 tggtcagcag ccacgcatca tccggggtgc gaataaaatg tttaatggtc ggcagcggca
     3241 taaattcggt cagccagttc agacgcacca tttcatcggt cacatcgttc gccacgctgc
     3301 ctttgccatg tttcagaaac agttccggcg catccggttt gccatacaga cgataaatgg
     3361 tcgcgccgct ctgacccacg ttatcacgcg cccatttata gccatacaga tccgcatcca
     3421 tgttgctgtt cagacgcgga cggctacagc tcgtttcacg ctgaatatgg ctcataacac
     3481 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
     3541 cttgtgcaat gtaacatcag agattttgag acacaaattt
//
